Curriculum Vitae






Jim C. Spain

Professor Emeritus
Environmental Engineering




Biography

Jim Spain joined the School of Civil and Environmental Engineering at the Georgia Institute of Technology at the beginning of 2005. Dr. Spain received his PhD in microbiology from The University of Texas at Austin and a BS in Biology from the University of Texas at Arlington. He studied the biodegradation of pesticides in the marine environment for five years as a post doctoral fellow and research scientist at the U.S. Environmental Protection Agency Marine Environmental Research Laboratory. Prior to joining Georgia Tech Dr. Spain directed the Environmental Biotechnology research program at the Air Force Research Laboratory in Panama City, Florida where he studied the biodegradation of synthetic organic compounds in the environment. His research interests in environmental biotechnology include: discovery and construction of bacteria for degradation of substituted aromatic compounds; physiological and ecological factors controlling microbial processes; and discovery of biocatalysts for green chemistry synthesis of novel materials. He works at the interface between basic microbiology research and practical applications to solve environmental problems.

Dr. Spain is a former editor for Applied and Environmental Microbiology and has published over 160 peer reviewed papers, several books, and numerous book chapters on the biodegradation and biosynthesis of organic compounds. He has served on review committees for the EPA, DoE, NIEHS, and DoD and on the editorial boards of a variety of journals.

Education

  • Ph.D., University of Texas at Austin, Microbiology and Biochemistry, 1979
  • B.S., University of Texas at Arlington, Biology and Chemistry, 1973

Research Interests

  • Environmental distribution, persistence, and biodegradation of chemical pollutants
  • Green chemistry synthesis of organic compounds by biocatalysis
  • Biodegradation pathways in bacteria for application to bioremediation
  • Environmental biotechnology related to marine, freshwater and subsurface ecosystems
  • Evolution and adaptation of microbial communities
  • Biochemistry, ecology, and molecular biology of environmentally relevant microbes
  • Discovery and characterization of bacteria that degrade synthetic organic compounds
  • Photobiological hydrogen production by cyanobacteria

Honors

  • 2011 Sir Fredrick McMaster Fellowship, CSIRO Australia Visiting Lectureship
  • 2009 American Society for Microbiology-Proctor and Gamble Award for Excellence in Environmental Microbiology
  • 1994 Elected Fellow American Academy for Microbiology

Patents

  • L.J. Nadeau, H. Luckarift, and J.C. Spain. 2011. Biocatalytic process for the production of ortho-aminophenols from chloramphenicol and analogs. US Patent No. 11,602,435.
  • Spain, J.C., L.J. Nadeau, and Z. He 2004. Biological process for the production of ortho-aminophenols from nitroaromatic compounds. US Patent No. 6,797,497. September 28, 2004.
  • He, Z., and J. C. Spain. 2002. Preparation of 2-aminomuconate from aminophenol by coupled enzymatic dioxygenation and dehydroxylation. US Patent No. 6,432,683. Aug. 13, 2002.
  • Spain, J.C., S.F. Nishino, and U. Lendenmann. Process for the biodegradation of dinitrotoluene. US Patent No. 6,248,580 B1. June 19, 2001.
  • Nadeau, L.J., J.C. Spain, and V. Kadiyala. 2005. Biological process for the conversion of nitroarenes to ortho-aminophenols using recombinant E. coli strains. US Patent No. 7,364,881. April 29, 2008.
  • Tan, L-S.,R. Kannan, J.C. Spain and L. Nadeau. 2006. Enzymatically Produced o-Aminophenol Containing AB-Monomer for Heterocyclic Rigid-Rod Polymer Synthesis. 7,495,106. Feb. 24, 2009.

Articles

  1. Orellana, L.H., J.K. Hatt, R. Iyer, K. Chourey, R.L. Hettich, J.C. Spain, W. H. Yang, J.C. Chee-Sanford, F.E. Loeffler and K.T. Konstantinidis. 2019. Multi-omic techniques predict the dynamics of microbial nitrogen utilization in agricultural soil. Sci. Rep. In Press.
  2. Palatucci, M.L., L.A. Waidner, E.E. Mack, and J.C Spain. 2019. Aerobic biodegradation of 2,3- and 3,4-dichloronitrobenzene. J. Haz. Mat. 10.1016/j.jhazmat.2019.05.110.
  3. Karthikeyan, S., L.M. Rodriquez, P. Heritier-Robbins, M. Kim, W.A. Overholt, J.C. Gaby, J.K. Hatt, J.C. Spain, R. Rossello-Mora, M. Huettel, J.E. Kostka, and K.T. Konstantinidis. 2019. Candidatus Macondimonas diazotrophica”, a novel gammaproteobacterial genus dominating crude oil-contaminated coastal sediments. Environ. Microbiol. https://doi.org/10.1038/s41396-019-0400-5
  4. Yu, R., Z. Kurt, F. He, and J.C. Spain. 2018. Biodegradation of the allelopathic chemical pterostilbene by a Sphingobium sp. strain from the peanut rhizosphere. Appl. Environ. Microbiol. DOI: 10.1128/AEM.02154-18
  5. Zhao, H., Y. Xu, S. Lin, J.C. Spain, N-Y. Zhou. 2018. The molecular basis for the NIH shift in carboxyl group migration. Molecular Microbiology. 110:411-424.
  6. Ulrich, B.A., M. Palatucci, J. Bolotin, J.C Spain and T.B. Hofstetter. 2018. Different mechanisms of alkaline and enzymatic hydrolysis of the insensitive munition component 2,4-dinitroanisole lead to identical products. Environ. Sci. Technol. Lett. DOI: 10.1021/acs.estlett.8b00258
  7. Kurt, Z., M. Minoia, and J.C. Spain. 2018. Resveratrol as a growth substrate for bacteria from the rhizosphere. Environ. Microbiol. 84: doi:10.1128/AEM.00104-18.
  8. Mundle, S.O.C, J.C. Spain, G.L. Lacrampe-Couloume, S.F. Nishino, and B.S. Lollar. 2017. Branched pathways in the degradation of cDCE by cytochrome P450 in Polaromonas sp. JS666. Sci. Tot. Environ. 15:99-105.
  9. Mahan, K.M., H. Zheng, T.T. Fida, R.J. Parry, D.E. Graham and J.C. Spain. 2017. A novel, iron-dependent enzyme that catalyzes the initial step in the biodegradation of N-nitroglycine by Variovorax sp strain JS1663. Appl. Environ. Microbiol. doi:10.1128/AEM.00457-17.
  10. Konstantinidis, K., Y. Wang, J. Hatt, D. Tsementzi, L. Rodriguez, C. Ruiz-Perez, M. Weigand, H. Kizer, G. Maresca, R. Krishnan, R. Poretsky, and J. C. Spain. 2017. Quantifying the importance of the rare biosphere for microbial community response to organic pollutants in a freshwater ecosystem. Appl. Environ. Microbiol. 83 (8) e03321-16.
  11. Kalyoncu, S., D.P. Heaner, Z. Kurt, C.M. Bethel, C.U. Ukachukwu, S. Chakravarthy, J.C. Spain, and R.L. Lieberman. 2016. Enzymatic hydrolysis by transition-metal-dependent nucleophilic aromatic substitution. Nature Chem. Biol. 12:1031-1036.
  12. Chen, Y., D. Hou, C. Lu, J.C. Spain, J. Luo. 2016. Effects of rate-limited mass transfer on modeling vapor intrusion with aerobic biodegradation. Environ. Sci. Technol. 50:9400-9406.
  13. Kurt. Z., E.E. Mack and J.C. Spain. 2016. Natural attenuation of non-volatile contaminants in the capillary fringe. Environ. Sci. Technol. 50:10172–10178.
  14. Chen, Y., D. Hou, C. Lu, J.C. Spain, J. Luo. 2016. Effects of rate-limited mass transfer on modeling vapor intrusion with aerobic biodegradation. Environ. Sci. Technol. 50:9400-9406.
  15. S Karthikeyan and J.C. Spain. 2016. Immobilized biocatalyst for detection and destruction of the insensitive explosive, 2,4-dinitroanisole (DNAN). Environ. Sci. Technol. 50:11193–11199.
  16. Yan, X., T. Gu, Z. Yi, J. Huang, X. Liu, J. Zhang, X. Xu, Z. Xin, Q. Hong, J. He, J.C. Spain, S. Li, and J. Jiang. 2016. Comparative genomic analysis of isoproturon-mineralizing sphingomonads reveals the isoproturon catabolic mechanism. Environ. Microbiol. DOI: 10.1111/1462-2920.13413
  17. S Karthikeyan and JC Spain. 2016. Biodegradation of 2,4-dinitroanisole (DNAN) by Nocardioides sp. JS1661 in water, soil and bioreactors. J. Haz. Materials 312:37-44.
  18. Salvi, F., J. Agniswamy, H. Yuan, K. Vercammen, R. Pelicaen, P. Cornelius, J.C. Spain, I.T. Weber, and G. Gadda. 2014. The combined structural and kinetic characterization of a bacterial nitronate monooxygenase from Pseudomonas aeruginosa PAO1 establishes NMO class I and II. J. Biol. Chem. 289(34):23764-75.
  19. Luo, J., Z. Kurt, D. Hou, and J.C. Spain. 2015. Modeling aerobic biodegradation in the capillary fringe. Environ. Sci. Technol. 49:1501-1510.
  20. Vercammen, K., Q. Wei, D. Charlier, A. Dötsch, S. Haussler, S. Schulz, F. Salvi, G. Gadda, J.C Spain, M. L. Rybtke, T. Tolker-Nielsen, J. Dingemans, L. Ye, and P. Cornelis. 2015. Pseudomonas aeruginosa LysR PA4203 regulator NmoR acts as a repressor of the PA4202 nmoA gene encoding a nitronate monooxygenase. J. Bacteriol. 197:1026-1039.
  21. Kurt, Z., E.E. Mack, and J.C. Spain. 2014. Biodegradation of cis-dichloroethene and vinyl chloride in the capillary fringe. Environ. Sci. Technol. 48, 13350−13357.
  22. Fida, T.T., S. Palamuru, G. Pandey, and J.C. Spain. 2014. Aerobic biodegradation of 2,4-dinitroanisole (DNAN) by Nocardioides sp. JS1661. Appl. Environ. Microbiol. 80:7725-31.
  23. Oh, S.,Z. Kurt, D. Tsementzi, M. Weigand, M. Kim, J. Hatt, M. Tandukar, S. Pavlostathis, J.C. Spain, and K. Konstantinidis. 2014. Microbial community degradation of widely used quaternary ammonium disinfectants. Appl. Environ. Microbiol. 80:5892-5900.
  24. Han, JI, JC Spain, JR Leadbetter, G. Ovchinnikova, LA Goodwin, CS Han, T Woyke, KW Davenport and P. Orwin. 2013. Genome of the root-associated plant growth-promoting bacterium Variovorax paradoxus strain EPS. Genome Announcements. 1:e00843-13.
  25. Francis, K., C. Smitherman, S. Nishino, J.C. Spain and G. Gadda. 2013. The biochemistry of the metabolic poison propionate 3-nitronate and its conjugate acid, 3-nitropropionate. IUBMB Life. 65:759-768.
  26. Wijker, RS, Z. Kurt, JC Spain, J. Bolotin, J. Zeyer and TB Hofstetter. 2013. Isotope fractionation associated with the biodegradation of 2- and 4-nitrophenols via monooxygenation pathways. Environ. Sci. Technol. 47:14185-14193.
  27. Anton BP, Chang Y-C, Brown P, Choi H-P, Faller LL, et al. 2013. The COMBREX project: design, methodology, and initial results. PLoS Biol. 11(8): e1001638.
  28. Wijker, R., J. Bolotin, S.N. Nishino, J.C. Spain, and T. Hofstetter. 2013. Using compound-specific isotope analysis to assess the biodegradation of nitroaromatic explosives in the subsurface. Environ. Sci. Technol. 47:6872-6883.
  29. Kurt, Z., and J.C. Spain. 2013. Biodegradation of chlorobenzene, 1,2-dichlorobenzene, and 1,4-dichlorobenzene in the vadose zone. Environ. Sci. Technol. 47:5846-5854.
  30. Nishino, S.F., K. Shin, J.G. Gossett, and J.C. Spain. 2013. Cytochrome P450 initiates degradation of cis-dichloroethylene by Polaromonas sp. JS666. Appl. Environ. Microbiol. 79:2263-2272.
  31. Parks, S.T., Y. Qu, and J.C. Spain. 2013. Identity and distribution of denA encoding 4-hydroxy-3-nitrophenylacetate denitrase, an FAD-dependent monooxygenase that catalyzes the key step involved in 3-nitrotyosine biodegradation in Variovorax sp. JS669. Microbiology. In revision.
  32. Kurt, Z., K. Shin, and J.C. Spain. 2012. Biodegradation of chlorobenzene and nitrobenzene at the oxic/anoxic interface between sediment and water. Environ. Sci. Technol. 46:11829-11835.
  33. Pati, S.G., K. Shin, M. Skarpeli-Liati, J. Bolotin, S.N. Eustis, J.C. Spain, and T.B. Hofstetter. 2012. Carbon and nitrogen isotope effects associated with the dioxygenation of aniline and diphenylamine. Environ. Sci. Technol. 46:11844-11853.
  34. Francis, K., S. Nishino, J.C. Spain and G. Gadda. 2012. A novel activity for fungal nitronate monooxygenase: detoxification of the metabolic inhibitor propionate-3-nitronate. Arch. Biochem. Biophys. 521:84-89.
  35. Husserl, J., J.B. Hughes, and J.C. Spain. 2012. Key enzymes enable growth of Arthrobacter strain JBH1 on nitroglycerin as the sole source of carbon and nitrogen. Appl. Environ. Microbiol. 78: 3649-3655.
  36. Coleman, N.V., N.L. Wilson, K. Barry, T.S. Brettin, D.C. Bruce, A. Copeland, E. Dalin, J.C. Detter, T.G. del Rio, L.A. Goodwin, N.M. Hammon, S. Han, L.J. Hauser, S. Israni, E. Kim, N. Kyrpides, M.L. Land, A. Lapidus, F. W. Larimer, S. Lucas, S. Pitluck, P. Richardson, J. Schmutz, R. Tapia, S.Thompson, H.N. Tice, J.C. Spain, J.G. Gossett, T.E. Mattes. 2011. Genome sequence of the ethene-and vinyl chloride-oxidizing actinomycete Nocardioides sp. strain JS614. J. Bacteriol. 193:3399.
  37. Hoffstetter, T.B, J. Bolotin, M. Skarpeli-Liati, J. Wijker, Z. Kurt, S.F. Nishino and J.C. Spain. 2011. Tracking transformation processes of organic micropollutants in aquatic environments using multi-element isotope fractionation analysis. Appl. Geochem. 26S:334-336.
  38. Pumphrey, G.M., A. Ranchou-Peyruse, and J.C. Spain. Cultivation-independent detection of autotrophic hydrogen-oxidizing bacteria by DNA stable isotope probing. 2011. Appl. Environ. Microbiol.77:4931-4938.
  39. Schroeder, S., A. Ranchou-Peyruse, M. Ranchou- Peyruse, and J.C. Spain. 2011. Reverse-transcriptase quantitative PCR method to detect interspecies hydrogen exchange by Alcaligenes hydrogenophilus, an aerobic hydrogen-oxidising bacterium. Arch. Microbiol. 193:687-692.
  40. Qu, Y., and J.C. Spain. 2011. Molecular and biochemical characterization of the 5-nitroanthranilic acid degradation pathway in a Bradyrhizobium. J. Bacteriol. 193:3057-3063.
  41. Parry, R., S. Nishino, and J.C. Spain. 2011. Naturally- occuring nitro compounds. Nat. Prod. Reports. 28:152-167.
  42. Qu, Y. and J.C. Spain. 2011. Catabolic pathway for 2-nitroimidazole involves a novel nitrohydrolase that also confers drug resistance. Environ. Microbiol. 13:1010-1017.
  43. Han, J., H.-K. Choi, S.-W. Lee, P.M. Orwin, J. Kim, S.L. LaRoe, T. Kim, J. O’Neil, J.R. Leadbetter, S.Y. Lee, C.-G. Hur, J.C. Spain, G. Ovchinnikova, L. Goodwin, and C. Han. 2011. Complete genome sequence of the metabolically versatile plant growth-promoting endophyte, Variovorax paradoxus S110. J. Bacteriol. 193:1183-1190.
  44. Nishino, S.F, K.A Shin, R. Payne, and J.C. Spain. 2010. Growth of bacteria on 3-nitropropionic acid as a sole carbon, nitrogen and energy source. Appl. Environ. Microbiol. 76:3590-3598.
  45. Qu, Y. and J.C. Spain. 2010. Biodegradation of 5-nitroanthranilic acid by Bradyrhizobium sp. Strain JS329. Appl. Environ. Microbiol. 76:1417-1422.
  46. Husserl, J. J.C. Spain, and J.B. Hughes. 2010. Growth of Arthrobacter sp. Strain JBH1 on nitroglycerin as the sole source of carbon and nitrogen. Appl. Environ. Microbiol. 76:1689-1691.
  47. Jennings, L.K., M.M.G. Chartrand, G. Lacrampe-Couloume, B.S. Lollar, J.C. Spain, and J.M. Gossett. 2009. Proteomic and transcriptomic analyses reveal genes upregulated by cis-dichloroethene in Polaromonas JS666. Appl. Environ. Microbiol. 75:3733-3744.
  48. Shin, K.E, and J.C. Spain. 2009. Biodegradation of diphenylamine by Burkholderia sp. Strain JS667: pathway and evolutionary implications. Appl. Environ. Microbiol. 75:2694-2704.
  49. Rankin, L.D., D.M. Bodenmiller, J.D. Partridge, S.F. Nishino, J.C. Spain, and S. Spiro. 2008. Escherichia coli NsrR regulates a pathway for oxidation of 3-nitrotyramine to 4-hydroxy-3-nitrophenylacetate. J. Bacteriol. 190:6170-6177.
  50. Hofstetter, T.B., J.C. Spain, S.F. Nishino, J. Bolotin, and R.P. Schwarzenbach. 2008. Identifying competing aerobic nitrobenzene biodegradation pathways by compound-specific isotope analysis. Environ. Sci. Technol. 42:4764-4770.
  51. Luckarift, H.R., and J.C. Spain. 2008. Continuous-flow applications of silica-encapsulated enzymes. Biomol. Catalysis. ACS Symposium Series. 986:243-253.
  52. Li, L. T.V. Marolla, L. Nadeau, and J.C. Spain. 2007. Probing the role of promoters in zinc reduction of nitrobenzene: continuous production of hydroxylaminobenzene. Ind. Eng. Chem. Res. 46:6840-6846.
  53. Betancor, L., H.R. Luckarift, J.H Seo, O. Brand and J.C. Spain. 2007. Three dimensional immobilization of β-galactosidase on a silicon surface. Biotechnol. Bioeng. 99:261-267.
  54. Luckarift, H.R., R. Greenwald, M. Bergin, J.C. Spain, and G.R. Johnson. 2007. Biosensor system for continuous monitoring of organophosphate aerosols. Biosens. Bioelectron. 23:400-406.
  55. Luckarift, H.R., Bosung Ku, J.C. Spain, and J. Dordick. 2007. Silica-immobilized enzymes for multi-step synthesis in microfluidic devices. Biotechnol. Bioeng. 98:701-705.
  56. Poulsen, N. C. Berne, J. Spain, and N. Kroger. 2007. Silica immobilization of an enzyme via genetic engineering of the diatom, Thalassiosira pseudonana. Angewandte Chemie. 46:1843-1846.
  57. Mattes, T.E., N.V. Coleman, A.S. Chuang, A.J. Rogers, J.C. Spain, and J.M. Gossett. 2006. Mechanism controlling the extended lag period associated with vinyl chloride starvation in Nocardioides sp. strain JS614. Arch. Microbiol. 187:217-226.
  58. Betancor, L., C. Berne, Luckarift, H.R., and J.C. Spain. 2006. Coimmobilization of a redox enzyme and a cofactor regeneration system. Chem. Comm. (34):3640-3642.
  59. Berne, C., L. Betancor, H.R. Luckarift, and J.C. Spain. 2006. Application of a microfluidic reactor for screening cancer prodrug activation using silica-immobilized nitrobenzene nitroreductase. Biomacromolecules. 7:2631-2636.
  60. He, Zhongqi, R.E. Parales, J.C. Spain, and G.R. Johnson. 2007. Novel organization of catechol meta pathway genes in the nitrobenzene degrader Comamonas sp. JS765 and its evolutionary implication. J. Ind. Microbiol. Biotechnol. 34:99-104.
  61. Luckarift, H.R., G.R. Johnson, and J.C. Spain. 2006. Silica-immobilized enzyme reactors; application to cholinesterase-inhibition studies. J. Chromatogr. B. 843:310-316.
  62. Wang, D.H, J-.B. Baek, S.F. Nishino, J.C. Spain, and L-.S. Tan. 2006. Thermally reactive phenylethynyl-terminated bis(benzylester) and bis(amide) monomers based on semi-enzymatically produced 6-phenylethynyl picolinic acid. Polymer. 47:1197-1206.
  63. Luckarift, H.R., M.B. Dickerson, K.H. Sandhage, and J.C. Spain. 2006. Rapid, room temperature synthesis of anti-bacterial bio-nano composites of lysozyme with amorphous silica or titania. Small. 2:640-642.
  64. Nadeau, L.J., J.C. Spain, R. Kannan, and L-S. Tan. 2006. Conversion of 2-(4-carboxyphenyl)-6-nitrobenzothiazole to 4-(6-amino-5-hydroxybenzothiazol-2-yl)benzoic acid by a recombinant E. coli strain. Chem. Comm. (5):564-565.
  65. Nishino, S.F., and J.C. Spain. 2006. Biodegradation of 3-nitrotyrosine by Burkholderia sp. JS165 and Variovorax paradoxus JS171. Appl. Environ. Microbiol. 72:1040-1044.
  66. Daprato, R.C., C. Zhang, J.C. Spain, and J.B. Hughes. 2005. Modeling aerobic bioremediation of 2,4-dinitrotoluene in a bioslurry reactor. Environ. Eng. Sci. 22:676-688.
  67. Johnson, G.R. and J.C. Spain. 2005. Synthesis of substituted catechols using nitroarene dioxygenases. Enzyme Microb. Technol. 38:142-147.
  68. Fournier, D., S. Trott, J.C. Spain, and J. Hawari. 2005. Metabolism of the aliphatic nitramine 4-nitro-2,4-diazabutanal (NDAB) by Methylobacterium sp. strain JS178. Appl. Environ. Microbiol. 71:4199-4202.
  69. Mattes, T.E., N.V. Coleman, J.C. Spain and J.M. Gossett. 2005. Physiological and molecular genetic analyses of vinyl chloride and ethene biodegradation in Nocardioides sp. strain JS614. Arch. Microbiol. 183:95-106.
  70. Luckarift, H.R., L.J. Nadeau, and J.C. Spain. 2005. Continuous synthesis of aminophenols from nitroaromatic compounds by combination of metal and biocatalyst. Chem. Comm. (3):383-384.
  71. Luckarift, H.R., G.R. Johnson, and J.C. Spain. 2004. Biosynthesis of hydroxydiphenylacetylene by regiospecific monooxygenation. Chem. Comm. (14):2402-2403.
  72. Naik, R.R., M.M. Tomczak, H.R. Luckarift, J.C. Spain, and M.O. Stone. 2004. Entrapment of enzymes and nanoparticles using biomimetically synthesized silica. Chem. Comm. (14):1684-1685.
  73. Zhao, J.-S., J.C. Spain, S. Thiboutot, G. Ampleman, C. Greer, and J. Hawari. 2004. Phylogeny of cyclic nitramine-degrading psychrophilic bacteria in marine sediment and their potential role in the natural attenuation of explosives. FEMS Microbiol. Ecol. 49:349-357.
  74. Bhushan B., A. Halasz, J. C. Spain, and J. Hawari. 2004. Initial reaction(s) in biotransformation of CL-20 catalyzed by salicylate 1-monooxygenase from Pseudomonas sp. Appl Environ. Microbiol. 70:4040-4047.
  75. Luckarift, H.R., J.C. Spain, R.R. Naik, and M.O. Stone. 2004. Enzyme immobilization in a biomimetic support. Nature Biotechnol. 22:211-213.
  76. Fournier, D., A. Halasz, J.C. Spain, R.J. Spanggord, J.C. Bottaro, and J. Hawari. 2004. Biodegradation of the RDX ring-cleavage product, 4-nitro-2,4-diazabutanal (NDAB) by Phanerochaete chrysosporium. Appl. Environ. Microbiol. 70:1123-1128.
  77. Fleischmann, T.J., K.C. Walker, J.C. Spain, J.B. Hughes, and A.M. Craig. 2004. Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes. Biochem. Biophys. Res. Comm. 314:957-963.
  78. Coleman, N.V. and J.C. Spain. 2003. Epoxyalkane:Coenzyme M transferase catalyzes epoxide metabolism during biodegradation of ethene and vinyl chloride in Mycobacterium strain JS60. J. Bacteriol. 185:5536-5545.
  79. Bhushan, B., L. Paquet, J.C. Spain, and J. Hawari. 2003. Biotransformation of 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane (CL-20) by denitrifying Pseudomonas sp. Strain FA1. Appl. Environ. Microbiol. 69:5216-5221.
  80. Fortner, J. D., C. Zhang, J. C. Spain, and J. B. Hughes. 2003. Soil column evaluation of factors controlling biodegradation of DNT in the vadose zone. Environ. Sci. Technol. 37:3382-3391.
  81. Coleman, N.V. and J.C. Spain. 2003. Distribution of the coenzyme M pathway of epoxide metabolism among ethene- and vinyl chloride-degrading mycobacteria: evidence for plasmid-mediated lateral gene transfer. Appl. Environ. Microbiol. 69:6041-6046.
  82. Zhao, J. S., J.C. Spain and J. Hawari. 2003. Phylogenetic and metabolic diversity of RDX transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source. FEMS Microbiol. Ecol. 46:189-196.
  83. Kadiyala, V., L.J. Nadeau, and J.C. Spain. 2003. Construction of E. coli strains for conversion of nitroacetophenones to ortho-aminophenols. Appl. Environ. Microbiol. 69:6520-6526.
  84. Johnson, G.R., and J.C. Spain. 2003. Evolution of catabolic pathways for synthetic compounds: bacterial pathways for degradation of 2, 4-dinitrotoluene and nitrobenzene. Appl. Microbiol. Biotechnol. 62:110-123.
  85. Spain, J.C., S.F. Nishino, B. Witholt, L.-S. Tan, and Wouter Duetz. 2003. Production of 6-phenylacetylene picolinic acid from diphenylacetylene by a toluene-degrading Acinetobacter. Appl. Environ. Microbiol. 69:4037-4042. 86. Bhushan, B., S. Trott, J. Spain, A. Halasz, and J. Hawari. 2003.
  86. Bhushan, B., S. Trott, J. Spain, A. Halasz, and J. Hawari. 2003. Biotransformation of RDX by cytochrome P450 2B4: Insights into the mechanism of RDX biodegradation by Rhodococcus sp.strain DN22. Appl. Environ. Microbiol. 69:1347-1351.
  87. Nadeau, L.J., Z. He, and J.C. Spain. 2003. Bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes involves intramolecular transfer of hydroxyl groups. Appl. Environ. Microbiol. 69:2786-2793.
  88. Muller, T.A., C. Werlen, J.C. Spain, and J.R. van der Meer. 2003. Evolution of a chlorobenzene degradative pathway among bacteria in a contaminated groundwater mediated by a genomic island in Ralstonia. Environ. Microbiol. 5:163-173.
  89. Bhushan, B., L. Paquet, A. Halasz, J.C. Spain, and J. Hawari. 2003. Mechanism of xanthine oxidase catalyzed biotransformation of HMX under anaerobic conditions. Biochem. Biophys. Res. Comm. 306:509-515.
  90. Trott, S., S.F. Nishino, J. Hawari, and J.C. Spain. 2003. Biodegradation of the nitramine explosive, CL-20. Appl. Environ. Microbiol. 69:1871-1874.
  91. Coleman, N.V., J.C. Spain, and T. Duxbury. 2002. Evidence that RDX biodegradation by Rhodococcus strain DN22 is plasmid-borne and involves a cytochrome p-450. J. Appl. Microbiol. 93:463-472.
  92. Coleman, N.V., T.E. Mattes, J.M. Gossett, and J.C. Spain. 2002. Phylogenetic and kinetic diversity of aerobic vinyl chloride-assimilating bacteria from contaminated sites. Appl. Environ. Microbiol. 68:6162-6171.
  93. Johnson, G.R., R.K. Jain, and J.C. Spain. 2002. Origins of the 2,4-dinitrotoluene pathway. J. Bacteriol. 184:4219-4232.
  94. Bhushan, B., A. Halasz, J. Spain, S. Thiboutot, G. Ampleman, and J. Hawari. 2002. Biotransformation of hexahydro-1,2,4-trinitro-1,3,5-triazine catalyzed by a NAD(P)H:nitrate oxidoreductase from Aspergillus niger. Environ. Sci. Technol. 36:3104-3108.
  95. Coleman, N.V., T.E. Mattes, J.M. Gossett, and J.C. Spain. 2002. Biodegradation of cis-dichloroethene as sole carbon source by a b-proteobacterium. Appl. Environ. Microbiol. 68:2726-2730.
  96. Bhushan, B., A. Halasz, J.C. Spain, and J. Hawari. 2002. Diaphorase catalyzed biotransformation of RDX via N-denitration mechanism. Biochem. Biophys. Res. Com. 296:779-784.
  97. Lessner, D.J., G.R. Johnson, R.E. Parales, J.C. Spain, and D.T. Gibson. 2002. Molecular characterization and substrate specificity analysis of nitrobenzene dioxygenase from Comamonas sp. strain JS765. Appl. Environ. Microbiol. 68:634-641.
  98. Fournier, D., A. Halasz, J. Spain, P. Fiurasek, and J. Hawari. 2002. Determination of key metabolites during biodegradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) with Rhodococcus sp. Strain DN22. Appl. Environ. Microbiol. 68:166-172.
  99. Halaz, A., J. Spain, L. Paquet, C. Beaulieu, and J. Hawari. 2002. Insights into the formation and degradation mechanisms of methylenedinitramine during the incubation of RDX with anaerobic sludge. Environ. Sci. Technol. 36:633-638.
  100. Johnson, G.R., B.F. Smets, and J.C. Spain. 2001.Oxidative transformations of aminodinitrotoluene isomers by multicomponent dioxygenases. Appl. Environ. Microbiol. 67:5460-5466.
  101. Zhang C., R.C. Daprato, S.F. Nishino, J.C. Spain, and J.B. Hughes. 2001. Remediation of dinitrotoluene contaminated soils from former ammunition plants: soil washing efficiency and effective process monitoring in bioslurry reactors. J. Haz. Mat. 87:139-154.
  102. Davis, J. K, Z. He, G. C. Paoli, L. J. Nadeau, C. C. Somerville, and J. C. Spain. 2000. Sequence analysis and initial characterization of two isozymes of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Appl. Environ. Microbiol. 66:2965-2971.
  103. He, Z., L. J. Nadeau, and J. C. Spain. 2000. Characterization of hydroxylaminobenzene mutase from pNBZ139 cloned from Pseudomonas pseudoalcaligenes JS45: a highly-associated sodium-dodecyl-sulfate-stable enzyme catalyzing an intramolecular transfer of hydroxyl group. Eur. J. Biochem. 267:1110-1116.
  104. He, Z., and J. C. Spain. 2000. One-step production of picolinic acids from 2-aminophenols catalyzed by 2-aminophenol 1,6-dioxygenase. J. Ind. Microbiol. Biotechnol. 25:25-28.
  105. He, Z., and J. C. Spain. 2000. Reactions involved in the lower pathway for degradation of 4-nitrotoluene by Mycobacterium strain HL 4-NT-1. Appl. Environ. Microbiol. 66:3010-3015.
  106. Johnson, G. R., R. K. Jain, and J. C. Spain. 2000. Properties of the trihydroxytoluene oxygenase from Burkholderia cepacia R34; an extradiol dioxygenase from the 2,4-dinitrotoluene pathway. Arch. Microbiol. 173:86-90.
  107. Nishino, S. F., G. Paoli, and J. C. Spain. 2000. Aerobic degradation of dinitrotoluenes and pathway for bacterial degradation of 2,6-dinitrotoluene. Appl. Environ. Microbiol. 66:2139-2147.
  108. Zhang, C., J.B. Hughes, S.F. Nishino, J.C. Spain. 2000. Slurry-phase biological treatment of 2,4- and 2,6-dinitrotoluene: role of bioaugmentation and effects of high dinitrotoluene concentrations. Environ. Sci.Technol. 34:2810-2816.
  109. Nadeau, L. J., He Z. and J. C. Spain. 2000. Production of 2-amino-5-phenoxyphenol from 4-nitrobiphenyl ether using nitrobenzene nitroreductase and hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes strain JS45. J. Ind. Microbiol. Biotechnol. 24:301-305.
  110. Davis, J. K., Z. He, C. C. Somerville, and J. C. Spain. 1999. Genetic and biochemical comparison of 2-aminophenol 1,6-dioxygenase of Pseudomonas pseudoalcaligenes JS45 to meta cleavage dioxygenases: divergent evolution of 2-aminophenol meta cleavage pathway. Arch. Microbiol. 172:330-339.
  111. Smets, B. F., R. G. Riefler, U. Lendenmann, and J. C. Spain. 1999. Kinetic analysis of simultaneous 2,4-dinitrotoluene (DNT) and 2,6-DNT biodegradation in an aerobic fluidized-bed biofilm reactor. Biotechnol. Bioeng. 63:642-653.
  112. He, Z., and J. C. Spain. 1999. Preparation of 2-aminomuconate from 2-aminophenol by coupled enzymatic dioxygenation and dehydrogenation reactions. J. Ind. Microbiol. Biotechnol. 23:138-142.
  113. He, Z., and J. C. Spain.1999. Comparison of the downstream pathways for degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45 (2-aminophenol pathway) and by Comamonas sp. JS765 (catechol pathway).1999. Arch. Microbiol. 171:309-316.
  114. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss. 1999. Chemoselective nitro group reduction and reductive dechlorination initiate degradation of 2-chloro-5-nitrophenol by Ralstonia eutropha JMP134. Appl. Environ. Microbiol. 65:2317-2323.
  115. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss. 1999. 3-Hydroxylaminophenol mutase from Ralstonia eutropha JMP134 catalyzes a Bamberger rearrangement. J. Bacteriol. 181:1444-1450.
  116. Nadeau, L. J., G. S. Sayler, and J. C. Spain. 1999. Oxidation of 1,1,1,-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) by Alcaligenes eutrophus A5. Arch. Microbiol. 171:44-49.
  117. Nishino, S. F., J. C. Spain, H. Lenke and H.-J. Knackmuss. 1999. Mineralization of 2, 4- and 2, 6-dinitrotoluene in soil slurries. Environ. Sci. Technol. 33:1060-1064.
  118. Haigler, B.E., G.R. Johnson, W-C. Suen, and J.C. Spain. 1999. Biochemical and genetic evidence for meta-ring cleavage of 2, 4, 5-trihydroxytoluene in Burkholderia sp strain DNT. J. Bacteriol. 181:965-972.
  119. van der Meer, J.R., C. Werlen, S. Nishino, and J.C. Spain. 1998. Evolution of a pathway for chlorobenzene metabolism leads to natural attenuation in a contaminated groundwater. Appl. Environ. Microbiol. 64:4185-4193.
  120. Kadiyala, V., and J.C. Spain. 1998. A two-component monooxygenase catalyzes both the hydroxylation of p-nitrophenol and oxidative release of nitrite from 4-nitrocatechol in Bacillus sphaericus JS905. Appl. Environ. Microbiol. 64:2479-2484.
  121. Goodall, J. L., S. M. Thomas, J. C. Spain, and S. W. Peretti. 1998. Operation of mixed-culture immobilized cell reactors for the metabolism of meta- and para-nitrobenzoate by Comamonas sp. JS46 and Comamonas sp. JS47. Biotechnol. Bioeng. 59:21-27.
  122. Kadiyala, V., B. F. Smets, K. Chandran, and J. C. Spain. 1998. High affinity p-nitrophenol oxidation by Bacillus sphaericus JS905. FEMS Microbiol. Lett. 166:115-120.
  123. He, Z., J. K. Davis, and J. C. Spain. 1998. Purification, characterization, and sequence analysis of 2-aminomuconic 6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180:4591-4595.
  124. He, Z., and J. C. Spain. 1998. A novel 2-aminomuconate deaminase in the nitrobenzene degradation pathway of Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 180:2502-2506.
  125. Spiess, T., F. Desiere, P. Fischer, J. C. Spain, H-.J. Knackmuss, and Hiltrud Lenke. 1998. A new 4-nitrotoluene degradation pathway in a Mycobacterium sp. strain. Appl. Environ. Microbiol. 64:446-452.
  126. Vorbeck, C., H. Lenke, P. Fischer, J. C. Spain, and H-.J. Knackmuss. 1998. Initial reductive reactions in aerobic microbial metabolism of 2,4,6-trinitrotoluene (TNT). Appl. Environ. Microbiol. 64:246-252.
  127. Lendenmann, U., J.C. Spain, and B.F. Smets. 1998. Simultaneous biodegradation of 2,4-dinitrotoluene and 2,6-dinitrotoluene in an aerobic fluidized bed biofilm reactor. Environ. Sci. Technol. 32:82-87.
  128. He, Z., and J.C. Spain. 1997. Studies on the catabolic pathway of degradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45: Removal of the amino group from 2-aminomuconic semialdehyde. Appl. Environ. Microbiol. 63:4839-4843.
  129. Spain, J.C. 1997. Synthetic chemicals with potential for natural attenuation. Bioremediation J. 1:1-9.
  130. Fiorella, P. D., and J.C. Spain. 1997. Transformation of 2,4,6-trinitrotoluene (TNT) by Pseudomonas pseudoalcaligenes strain JS45. Appl. Environ. Microbiol. 63:2007-2015.
  131. Shah, M.M., and J.C. Spain. 1996. Elimination of nitrite from the explosive 2,4,6-trinitrophenylmethylnitramine catalyzed by ferredoxin NADP oxidoreductase from spinach. Biochem. Biophys. Res. Commun. 220:563-568.
  132. Lendenmann, U., and J.C. Spain. 1996. 2-Aminophenol 1,6-dioxygenase: a novel aromatic ring cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45. J. Bacteriol. 178:6227-6232.
  133. Suen, W.C., B.E. Haigler, and J.C. Spain. 1996. 2,4-Dinitrotoluene dioxygenase from Burkholderia sp. strain DNT: similarity to naphthalene dioxygenase. J. Bacteriol. 178:4926-4934.
  134. Haigler, B.E., W.C. Suen, and J.C. Spain. 1996. Purification and sequence analysis of 4-methyl-5-nitrocatechol oxygenase from Burkholderia sp. strain DNT. J. Bacteriol. 178:6019-6024.
  135. Haigler, B.E., and J.C. Spain. 1996. Degradation of nitroaromatic compounds by microbes. SIM News Feature Article. 46:59-68.
  136. Klecka, G., S. McDaniel, P. Wilson, C. Carpenter, J. Clark, A. Thomas, and J.C. Spain. 1996. Field evaluation of a granular activated carbon fluid-bed bioreactor for treatment of chlorobenzene in groundwater. Environ. Prog. 15:93-107.
  137. Somerville, C.C., S.F. Nishino, and J.C. Spain. 1995. Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes. JS45. J. Bacteriol. 177:3837-3842.
  138. Nishino, S.F., and J. C. Spain. 1995. Oxidative pathway for the biodegradation of nitrobenzene by Comamonas sp. strain JS765. Appl. Environ. Microbiol. 61:2308-2313.
  139. Nadeau, L.J., and J.C. Spain. 1995. Bacterial degradation of 3-nitrobenzoic acid. Appl. Environ. Microbiol. 61:840-843.
  140. An, D., D.T. Gibson, and J.C. Spain, 1994. Oxidative release of nitrite from 2-nitrotoluene by a three-component enzyme from Pseudomonas sp. strain JS42. J. Bacteriol. 176:7462-7467.
  141. Jain, R.K., J.H. Driesbach, and J.C. Spain, 1994. Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Appl. Environ. Microbiol. 60:3030-3032.
  142. Haigler, B.E., W.H. Wallace, and J.C. Spain, 1994. Biodegradation of 2-nitrotoluene by Pseudomonas sp. strain JS42. Appl. Environ. Microbiol. 60:3466-3469.
  143. Nishino, S.F., J.C. Spain, and C.A. Pettigrew, 1994. Biodegradation of chlorobenzene by indigenous bacteria. Environ. Toxicol. Chem. 13:871-877.
  144. Haigler, B.E., S.F. Nishino, and J.C. Spain, 1994. Biodegradation of 4-methyl-5-nitrocatechol by Pseudomonas sp. strain DNT. J. Bacteriol. 176:3433-3437.
  145. Nishino, S.F., and J.C. Spain, 1993. Degradation of nitrobenzene by a Pseudomonas pseudoalcaligenes. Appl. Environ. Microbiol. 59:2520-2525.
  146. Haigler, B.E., and J.C. Spain, 1993. Biodegradation of 4-nitrotoluene by Pseudomonas sp. strain 4NT. Appl. Environ. Microbiol. 59: 2239-2244.
  147. Suen, W.C., and J.C. Spain, 1993. Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation. J. Bacteriol. 175:1831-1837.
  148. Nishino, S.F., and J.C. Spain, 1993. Cell density-dependent adaptation of Pseudomonas putida to biodegradation of p-nitrophenol. Environ. Sci. Technol. 27:489-94.
  149. Haigler, B.E., C.A. Pettigrew, and J.C. Spain, 1992. Biodegradation of mixtures of substituted benzenes by Pseudomonas sp. strain JS 150. Appl. Environ. Microbiol. 58:2237-2244.
  150. Nishino, S.F., J.C. Spain, L.A. Belcher, and C.D. Litchfield, 1992. Chlorobenzene degradation by bacteria isolated from contaminated groundwater. Appl. Environ. Microbiol. 58:1719-1726.
  151. Ross, D.D., H. Mayfield, and J.C. Spain, 1992. Environmental fate and effects of jet fuel JP-8. Chemosphere. 24:219-228.
  152. Robertson, J.B., J.C. Spain, J.D. Haddock, and D.T. Gibson, 1992. Oxidation of nitrotoluenes by toluene dioxygenase: evidence for a monooxygenase reaction. Appl. Environ. Microbiol. 58:2643-2648.
  153. Spanggord, R.J., S.F. Nishino, and J.C. Spain, 1991. Biodegradation of 2,4-dinitrotoluene by a Pseudomonas sp. Appl. Environ. Microbiol. 57:3200-3205.
  154. Haigler, B.E., and J.C. Spain, 1991. Biotransformation of nitrobenzene by bacteria containing toluene degradative pathways. Appl. Environ. Microbiol. 57:3156-3162.
  155. Spain, J.C. and D.T. Gibson. 1991. Metabolic pathway for biodegradation of p-nitrophenol in Moraxella. Appl. Environ. Microbiol. 57:812-819.
  156. Pettigrew, C.A., B.E. Haigler, and J.C. Spain. 1991. Simultaneous biodegradation of toluene and chlorobenzene by Pseudomonas sp. strain JS6. Appl. Environ. Microbiol. 57:157-162.
  157. Spain, J.C., G.J. Zylstra, C.K. Blake, and D.T. Gibson. 1989. Monohydroxylation of phenol and 2,5-dichlorophenol by toluene dioxygenase in Pseudomonas putida F1. Appl. Environ. Microbiol. 55:2648-2652.
  158. Haigler, B.E. and J.C. Spain. 1989. Degradation of p-chlorotoluene by a mutant of Pseudomonas sp. strain JS6. Appl. Environ. Microbiol. 55:372-379.
  159. Spain, J.C. and D.T. Gibson. 1988. Oxidation of substituted phenols by Pseudomonas putida F1 and Pseudomonas sp. JS6. Appl. Environ. Microbiol. 54:1399-1404.
  160. Spain, J.C., D.C. Downey, and J. Milligan. 1989. Excessive bacterial decomposition of H2O2 during enhanced biodegradation. Groundwater. 27:163-167.
  161. Haigler, B.E., S.F. Nishino, and J.C. Spain. 1988. Degradation of 1,2-dichlorobenzene by a Pseudomonas sp. Appl. Environ. Microbiol.54:294-301.

Books

  1. Jennings, L.K., C.G.S. Giddings, J.M. Gossett, and J.C. Spain. 2013. Bioaugmentation for aerobic degradation of cis-1,2-dichloroethylene. In Stroo, H.F., A. Leeson, and C.H. Ward (eds). Bioaugmentation for Groundwater Remediation. SERDP ESTCP Environmental Remediation Technology. Volume 5:199-217.
  2. Nishino, S. F., and J. C. Spain. 2004. Catabolism of nitroaromatic compounds. pp. 575-608. In: J.-L. Ramos (ed.), The Pseudomonads Vol III. Biosynthesis of macromolecules and molecular metabolism. Kluwer Academic/Plenum Publishers.
  3. Ward, C.H., J.B. Hughes, G.A. Pope, M. Delshad, V. Dwaranath, J. Spain, S. Nishino, J.S. Fruchter, V.R. Vermeul, M.D. Williams, and J.E. Szecsody. 2002. In situ treatment technologies. In: D. Reible and K. Demnerova (eds.) Innovative Approaches to the On-Site Assessement and Remediation of Contaminated Sites. Kluwer Acacemic Publishers, Dordrecht, The Netherlands.
  4. Nishino, S.F. and J.C. Spain. 2001. Identification of bottlenecks to the in situ bioremediation of dinitrotoluene. In: Magar, V.S., G. Johnson, S.K. Ong, and A. Leeson (eds.) Bioremediation of Energetics, Phenolics, and Polycyclic Aromatic Hydrocarbons. Battelle Press, Columbus, OH.
  5. Nishino, S.F. and J.C. Spain. Biodegradation, transformation and bioremediation of nitroaromatic compounds. 2002. In: C.H. Hurst, G.R. Knudsen, M.J. McInerney, L.D. Stetzenbach, and M.V. Walter (eds.) Manual of Environmental Microbiology, 2nd Edition. ASM Press, Washington DC.
  6. Herrington, R.T., J. Hicks, D. Downey, J.C. Spain, S.F. Nishino, J. Gossett, and E. Becvar. 2000. Natural attenuation of chlorinated benzenes at a former disposal site. In: G.B. Wickramanayake, A.R. Gavaskar, and M.E. Kelley (eds.) Natural Attenuation Considerations and Case Studies: Remediation of Chlorinated and Recalcitrant Compounds. Battelle Press, Columbus, OH.
  7. Nishino, S. F., J. C. Spain and Z. He. 2000. Strategies for aerobic degradation of nitroaromatic compounds: process discovery to field application. In Spain, J. C., J. B. Hughes, and H. -J. Knackmuss (eds.). Biodegradation of Nitroaromatic Compounds and Explosives. Lewis Publishers, Boca Raton, FL
  8. Spain, J. and S. Nishino. 2000. Oxidative pathways for biodegradation of nitroaromatic compounds. Proceedings of the Ninth European Congress on Biotechnology. Branche Belge de la Societe de Chimie Industrielle, Brussels, Belgium.
  9. Spain, J. C., S. F. Nishino, M. R. Green, J. E. Forbert, N. A. Nogalski, R. Unterman, W. M. Riznychok, S. E. Thompson, P. M. Sleeper, and M. A. Boxwell. 1999. Field demonstration of FBR for treatment of nitrotoluenes in groundwater. In: B. C. Alleman and A. Leeson (eds.) Bioremediation of Nitroaromatic and Haloaromatic Compounds. Battelle Press, Columbus, OH.
  10. Ward, C. H., M. Alexander, J. Ryan, and J. C. Spain. 1999. Transformation. In: W. C. Anderson, R. C. Loehr, and B. P. Smith (eds), Environmental Availability in Soils. American Academy of Environmental Engineers, Wash. DC.
  11. Nishino, S. F., and J. C. Spain. 1999. Strategies for aerobic biodegradation of dinitrotoluenes. In R. Fass, .Y. Flashner, and S. Reuveny (eds.) Novel Approaches for Bioremediation of Organic Pollution. Plenum Press, New York.
  12. Nishino, S.F. and J.C. Spain, 1996. Biodegradation and transformation of nitroaromatic compounds. In C.H. Hurst, G.R. Knudsen, M.J. McInerney, L.D. Stetzenbach, and M.V. Walter (eds.) Manual of Environmental Microbiology. ASM Press, Washington, DC.
  13. Spain, J.C., 1995. Biodegradation of nitroaromatic compounds. In: L.N. Ornston, A. Ballows, and E.P. Greenberg (Eds.) Annual Reviews in Microbiology. Vol. 49:523-549. Annual Reviews Inc. Palo Alto, CA.
  14. Spain, J.C., 1995. Bacterial degradation of nitroaromatic compounds under aerobic conditions. In J.C. Spain (ed.). Biodegradation of nitroaromatic compounds. Plenum Press, New York.
  15. Spain, J.C., C.A. Pettigrew, B.E. Haigler, 1991. Biodegradation of mixed solvents by a strain of Pseudomonas. In: G.S. Sayler et al (eds). Environmental Biotechnology for Waste Treatment. Plenum Press, New York.
  16. Spain, J.C., 1990. Microbial adaptation in aquatic ecosystems. In: K.D. Racke and J.R. Coates (eds). Enhanced Biodegradation Pesticides in the Environment. American Chemical Society, Washington, D.C.
  17. Spain, J.C., 1990. Metabolic pathways for biodegradation of chlorobenzenes. In: S. Silver et al (eds). Pseudomonas: Biotransformations, Pathogenesis, and Evolving Biotechnology. American Society for Microbiology, Washington, D.C.
  18. Spain, J.C., 1988. Development of bacteria for biodegradation of chloroaromatic compounds. In: G. Lewandowski et al (eds). Biotechnology Applications in Hazardous Waste Treatment. Engineering Foundation, New York.

Proceedings

  1. Nishino, S.F., S. Craven, E. Lang, and J.C Spain. 2011. Biodegradation of m-tyrosine by rhizosphere bacteria. 111th General Meeting of the American Society for Microbiology
  2. Smitherman, C., K. Francis, S. Nishino, JC Spain, and G. Gadda. 2011. Detoxification of a lethal toxin by nitronate monooxygenase. International Symposium on Flavins and Flavoproteins, Berkeley, CA.
  3. Gadda, G., K. Francis, S. Nishino, and JC Spain. 2011. Propionate-3-nitronate oxidation by fungal nitronate monooxygenase: a 60- year mystery solved. Gordon Research Conference on Enzymes, Coenzymes and Metabolic Pathways.
  4. Wijker, R.S Zohre Kurt, Jim C. Spain, Jakov Bolotin, and Thomas B. Hofstetter. 2011. Enzyme kinetics of flavin-dependent monooxygenases modulate the observable isotope fractionation during monooxidation of 4-nitrophenol. American Chemical Society Symposium on Reaction Mechanisms in Environmental Organic Chemistry. Denver, CO.
  5. Cox, E., C. Austrins, J. Spain, K. Shin, S. Nishino, J. Gossett, C. Giddings, L. Jennings, E. Edwards, T. Johnson, M. Duhamel, and B. Sherwood-Lollar. 2010. The truth is out there: unraveling the mystery of the missing DCE, vinyl chloride, and ethane. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey, CA.
  6. Sherwood-Lollar, B., J. McKelvie, M. Elsner, S. Mancini, T. Johnson, M. Simpson, A. Simpson, E. Edwards, J. Gossett, L. Jennings, J.C Spain, and E. Cox. 2010. New developments in forensic evaluation of contaminant sources and microbial degradation pathways using CSIA. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey CA.
  7. Wan Johari, W.L., J.C. Spain, and J.M. Gossett. 2010. The involvement of a cyclohexanone monooxygenase gene of Polaromonas sp. JS666 during aerobic cis-dichloroethene degradation. Seventh International Conference on Remediation of Chlorinated and Recalcitrant Compounds, Monterey CA.
  8. Major, D., C. Aziz, J.M. Gossett, J.C. Spain, and B. Sherwood-Lollar. 2009. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP. Washington, D.C.
  9. Cox, E., J.M. Gossett, J.C. Spain, E. Edwards, and B. Sherwood-Lollar. 2009. The truth is out there: unraveling the mystery of the missing DCE, vinyl chloride, and ethane. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  10. Hofstetter, T.B, A.E. Hartenbach, J.C. Spain, S.F. Nishino, and R.P. Schwarzenbach. 2009. Using compound-specific carbon and nitrogen isotope analysis for the assessment of nitroaromatic compound transformation. American Chemical Society National Meeting, Salt Lake City.
  11. Qu, Yi and J.C. Spain. 2009. Biodegradation of 5-nitroanthranilic acid by a Bradyrhizobium sp. Annual Meeting of the American Society for Microbiology.
  12. Shin, Kwanghee and J.C. Spain. 2009. Biodegradation of diphenylamine by Burkholderia sp, JS667. Annual Meeting of the American Society for Microbiology.
  13. Shin, Kwanghee and J.C. Spain . 2009. Biodegradation of nitrobenzene, aniline, and diphenylamine at the oxic/anoxic interface between sediment and water. RemTec Remediation Technology Summit.
  14. Cox, E., C. Austrins, J. Gossett, J. Spain, E. Edwards, and B. Sherwood-Lollar. 2008. Elucidation of the mechanisms and environmental relevance of cis-dichloroethene and vinyl chloride biodegradation. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  15. Major, D., J. Gossett, L. Jennings, C. Sausville-Giddings, S. Nishino, J. Spain, C. Aziz, and M. Watling. 2008. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  16. Nishino, S.F, R.B. Payne, and J.C. Spain. 2008. Pathway for bacterial degradation of 3-nitropropionic acid and the purification and characterization of a novel denitrase. Annual Meeting of the American Society for Microbiology.
  17. Jennings, L.K., S.F. Nishino, R.B. Payne, J.C. Spain, and J.M. Gossett. 2008. Proteomic and transcriptomic analyses reveal genes upregulated by cis-dichloroethene in Polaromonas sp. JS666. Annual Meeting of the American Society for Microbiology.
  18. Shin, K. and J.C. Spain. 2007. Biodegradation of diphenylamine. Annual Meeting of the American Society for Microbiology.
  19. Seo, J.H, L. Betancor, K. S. Demirci, J.C Spain, and O. Brand. 2007. Liquid-Phase biochemical sensing with disk-type resonant microsensor. Transducers- 14th International Conference on Solid-State Sensors, Actuators, and Microsystems, Lyon, France.
  20. Kroeger, N., N.C. Poulsen, and J.C. Spain. 2007. Biogenesis of functional silica nanostructures through diatom genetic engineering. Society for Industrial Microbiology, Annual Meeting.
  21. Jennings, L. K., J. M. Gossett, and J. C. Spain. 2007. Deciphering cDCE degradation pathways in Polaromonas sp. strain JS666. Ninth International In- Situ and On-Site Bioremediation Symposium, Baltimore MD.
  22. Aziz, C.E., M. Watling, D. Major, L.K. Jennings, C.G. Sausville-Giddings, J.M. Gossett, S. Nishino and J.C. Spain. 2007. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology - Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  23. Aziz, C.E., M. Watling, D. Major, L.K. Jennings, C.G. Sausville-Giddings, J.M. Gossett, S. Nishino and J.C. Spain. 2006. Enhancing natural attenuation through bioaugmentation with aerobic bacteria that degrade cis-1,2-DCE. Partners in Environmental Technology - Technical Symposium & Workshop, SERDP/ESTCP, Washington, D.C.
  24. H.R. Luckarift, G.R. Johnson, M.M Tomczak, R.R. Naik, and J.C. Spain. 2006. Biomimetic silica encapsulation: an efficient and versatile enzyme immobilization technique. AIChE Annual Meeting, Bionanotechnology Symposium.
  25. Goldstein, K., R. Deeb, R. O’Laskey, M.C. Cavanaugh, J. Spain, J. Hughes, F. Bogle, S. Muffler, and L. Haines. 2006. Alternative closure strategy in karst setting at VAAP. Battelle-Fifth International Conference on Remediation of Chlorinated and Recalcitrant Compounds.
  26. Wang, D.H., J.-B. Baek, S.F. Nishino, J.C. Spain and L-.S. Tan. 2006. Semi-enzymatically produced 6-phenylethynyl picolinic acid as endcapping agent: Synthesis and thermal properties of phenylethynyl-terminated bis(benzylester) and bis(amide) monomers. Annual Meeting of the American Chemical Society. Polymer Preprints 47(1), 266-267.
  27. Nadeau, L.J., J.C. Spain, R. Kannan, and L-S. Tan. 2005. Nitroreductase and mutase produce a novel aminophenolic polymer synthon. Annual Meeting of the American Society for Microbiology.
  28. Eby, D.M., and J.C. Spain. 2005. Ring fission of 3-methyl-4-nitrocatechol during 2,6-dinitrotoluene degradation is catalyzed by a novel extradiol dioxygenase. Annual Meeting of the American Society for Microbiology.
  29. Johnson, G.R., D. M. Eby, and J. C. Spain. 2005. Transformation of aminomethylnitrocatechol isomers - towards an engineered pathway for aerobic 2,4,6-trinitrotoluene degradation. Annual meeting of the American Society for Microbiology.
  30. Berne, C., and J.C. Spain. 2005. Purification and characterization of hydroxylaminobenzene mutases A and B from Pseudomonas pseudoalcaligenes JS45. Annual meeting of the American Society for Microbiology.
  31. Mattes, T.E., N.V. Coleman, J.C. Spain, and J.M. Gossett. 2005. Characterization of the extended lag period associated with vinyl chloride and ethene starvation in Nocardioides sp. strain JS614. Annual meeting of the American Society for Microbiology.
  32. Nishino, S.F., and J.C. Spain. 2004. Biodegradation of 3-nitrotyrosine by Variovorax paradoxus strain JS171 and Burkholderia sp. JS165. Annual meeting of the American Society for Microbiology.
  33. Johnson, G.R., and J.C. Spain. 2004. Synthesis of novel catechols using bacterial nitroarene dioxygenases. Annual meeting of the American Society for Microbiology.
  34. H.R. Luckarift and J.C. Spain. 2005. A microbial 'toolbox' for the biosynthesis of novel polymer synthons from diphenylacetylene. Annual meeting of the American Society for Microbiology
  35. Mattes, T.E., J.M. Gossett, N.V. Coleman, and J.C. Spain. 2004. A linear plasmid carries vinyl chloride pathway genes in Nocardioides strain JS614. Battelle International Symposium.
  36. Naik, R.R., H.R. Luckarift, J.C. Spain, and M. Stone. 2004. Biomimetic encapsulation of enzymes. Annual Meeting of the Materials Research Society.
  37. Mattes, T.E., J.M. Gossett, N.V. Coleman, and J.C. Spain. 2003. Characterization of vinyl chloride and ethane starvation physiology in VC-assimilating Nocardioides strain JS614. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  38. Fleischmann, T.J., S.L. Ivey, K.C. Walker, J.C. Spain, and A.M. Craig. 2003. Anaerobic transformation of 2,4,6-TNT by bovine ruminal microbes. Annual meeting of the American Society for Microbiology.
  39. Mattes, T.E., N.V. Coleman, J.C. Spain, and J.M. Gossett. 2003. Evidence that vinyl chloride monooxygenase genes are encoded by a megaplasmid in Nocardioides strain JS614. Annual meeting of the American Society for Microbiology.
  40. Kachel, C.A, R. Giannone, R.E. Parales, G.R. Johnson, J.C. Spain, and G.J. Zylstra. 2003. Nucleotide Sequence Analysis of the Burkholderia sp. strain DNT Large catabolic plasmid for dinitrotoluene degradation. Annual meeting of the American Society for Microbiology.
  41. Nadeau, L.J., Z. He, and J.C. Spain. 2002. Intramolecular transfer of hydroxyl groups during bacterial conversion of hydroxylamino aromatic compounds by both lyase and mutase enzymes. Annual meeting of the American Society for Microbiology.
  42. Trott, S., J. Hawari, and J.C. Spain. 2002. Biodegradation of the cyclic nitramine explosive CL-20. Annual meeting of the American Society for Microbiology.
  43. Coleman, N. and J. Spain. 2001. Aerobic biodegradation of cis-dichloroethylene as sole carbon source by a beta-proteobacterium, strain JS666. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  44. Nishino, S.F., J.C. Spain, W.A. Duetz, and B. Witholt. 2001. Production of 6-phenylacetylene picolinic acid from diphenylacetylene by a toluene-degrading Acinetobacter. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  45. Paoli, G.C. and J.C. Spain. 2001. Solubilization and purification of a membrane associated hydroxylaminobenzene mutase. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  46. Johnson, G.R., B.F. Smets, and J.C. Spain. 2001. Oxidative transformation of aminodinitrotoluene isomers by multicomponent dioxygenases. ASM International Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico.
  47. DaPrato, R.C., C. Zhang, S.F. Nishino, J.C. Spain, and J.B. Hughes. 2001. Interpretation and modeling of slurry reactor performance to provide monitoring strategies for the aerobic bioremediation of dinitrotoluene contaminated soils. NATO Advanced Study Institute. Prague, Czech Republic.
  48. Zhang, C., J.B. Hughes, R. Deprato, S.F. Nishino, and J.C. Spain. 2001. Stoichiometry of dinitrotoluene mineralization in a pilot-scale slurry-phase bioreactor system. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  49. S.M. Cuffin, P.M. Lafferty, P.N. Taylor, J.C. Spain, and S.F. Nishino. 2001. Bioremediation of dinitrotoluene isomers in the unsaturated/saturated zone. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  50. Johnson, G.R., P.C. Gilcrease and J.C. Spain. 2000. Biocatalytic synthesis of polymer synthons using multicomponent toluene monooxygenases. Annual meeting of the American Society for Microbiology.
  51. Nishino, S.F. and J.C. Spain. 2000. In situ biodegradation of 2,4- and 2,6-dinitrotoluene. Annual Meeting American Society for Microbiology.
  52. Deeb, R. A., S. F. Nishino, J. C. Spain, H. Y. Hu, K. Scow, and L. Alvarez-Cohen. 2000. MTBE and benzene biodegradation by a bacterial isolate via two independent monooxygenase-initiated pathways. Annual Meeting American Chemical Society.
  53. J.C. Spain, S.F. Nishino, C. Zhang, and J.B. Hughes. 1999. DNT degradation in soil from Badger Army Ammunition Plant. Partners in Environmental Technology Technical Symposium and Workshop.
  54. Johnson, G.R. and J. C. Spain. 1999. Evolutionary origins of the pathway for 2,4-dinitrotoluene degradation. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  55. Paoli, G.C., S. F. Nishino and J. C. Spain. 1999. Metabolism of 3-methyl-4-nitrocatechol by 2,6-dinitrotoluene degrading bacteria. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  56. Nishino, S.F. and J.C. Spain. 1999. 2,4-DNT degradation in soil from Badger Army Ammunition Plant. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  57. Zhang, C., S. F. Nishino, J. B. Hughes, and J. C. Spain. 1999. Aerobic biodegradation of 2,4-dinitrotoluene and 2,6-dinitrotoluene in a pilot-scale sequential slurry reactor system. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives.
  58. Nadeau, L. J., Zhongqi He, and J. C. Spain. 1999. The biosynthesis of 2-amino-5-phenoxyphenol from 4-nitrobiphenyl ether using two enzymes, a nitroreductase and hydroxylaminobenzene mutase, from Pseudomonas pseudoalcaligenes JS45. Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives
  59. Deeb, R. A., J. C. Spain, and L. Alvarez-Coehn. 1999. Mineralization of benzene, toluene, ethylbenzene, m-xylene, and p-xylene by two Rhodococcus species. Annual Meeing American Society for Microbiology.
  60. He, Z., L. Nadeau, and J. C. Spain. 1999. Characterization of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  61. Deeb, R. A., J. C. Spain, and L. Alvarez-Coehn. 1999. Mineralization of BTEX compounds and MTBE in pollutant mixtures by enriched and pure microbial cultures. Battelle International Symposium on In-Situ and On-Site Bioremediation.
  62. Spain, J.C., and J.C. Cornette. 1998. Biotreatment of nitroaromatic compounds. US/Canada/UK TTCP Workshop on Environmental Aspects of Energetic Materials. Quebec, Canada.
  63. Spain, J.C. 1998. Biotreatment of dinitrotoluenes from munitions manufacturing sites. US/Germany Environmental Technology Data Exchange Meeting Johnson, G.R., B.E. Haigler, R.K. Jain, and J.C. Spain. 1998. Cloning and characterization of genes encoding enzymes for degradation of 2,4-dinitrotoluene from Burkholderia cepacia R34. Annual Meeting American Society for Microbiology.
  64. He, Z., J.K. Davis, and J.C. Spain. 1998. Purification, characterization, and sequence analysis of 2-aminomuconic-6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  65. Spain, J.C., S.F. Nishino, C. Werlen, and J.R. van der Meer. 1998. Evolution of a pathway for chlorobenzene metabolism in a contaminated ecosystem. Annual Meeting American Society for Microbiology.
  66. Nishino, S.F., and J.C. Spain. 1998. Mineralization of 2,4- and 2,6-dinitrotoluene in soil slurries by pure cultures of DNT-degrading bacteria. Annual Meeting American Society for Microbiology.
  67. Smets, B.F., J.C. Spain, and R.J. Mueller. 1998. Desorption and aerobic biodegradation of dinitrotoluenes in aged contaminated soils. Battelle Conference on Remediation of Chlorinated and Recalcitrant Compounds.
  68. Spiess, Tillman, Frank Desiere, Jim Spain, Hans-Joachim Knackmuss, and Hiltrud Lenke, 1997. Degradation of 4-nitrotoluene by a Mycobacterium sp. strain. Annual Meeting of the German Association of Microbiology-Hamburg.
  69. Davis, John K., C.C. Somerville, and J.C. Spain, 1997. Pseudomonas pseudoalcaligenes JS45 possesses two genes encoding hydroxylaminobenzene mutase. Annual Meeting American Society for Microbiology.
  70. Davis, John K., C.C. Somerville, and J.C. Spain, 1997. Cloning and characterization of the genes for 2-aminophenol 1,6-dioxygenase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  71. Lendenmann, Urs, B.F. Smets, and J.C. Spain, 1997. Biodegradation of 2,4-DNT and 2,6-DNT in a fluidized bed reactor with a mixed bacterial culture. Annual Meeting American Society for Microbiology.
  72. Venkateswarlu, K. and J.C. Spain, 1997. Nitrophenol biodegradation by Bacillus sphaericus JS905 expressing a soluble monooxygenase. Annual Meeting American Society for Microbiology.
  73. He, Z. and J.C. Spain, 1997. Final steps in the pathway for biodegradation of nitrobenzene by Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  74. Johnson, G.R., and J.C. Spain, 1997. Phylogenetic analysis of nitrotoluene dioxygenases. Annual Meeting American Society for Microbiology.
  75. Haigler, B.E., C.C. Somerville, and J.C. Spain, 1997. Sequence analysis differences between the 2,4-dinitrotoluene degrading genes of Burkholderia sp. strain R34 and strain DNT. Annual Meeting American Society for Microbiology.
  76. Nishino, S.F., and J.C. Spain, 1997. Ring cleavage of 3-methyl-4-nitrocatechol by 2,6-dinitrotoluene degrading bacteria. Annual Meeting American Society for Microbiology.
  77. Thomas, A., G. Klecka, and J.C. Spain, 1996. Treatment of chlorobenzene contaminated groundwater using a fluidized bed bioreactor. Engineering Foundation Conference on Bioremediation of Surface and Subsurface Contamination.
  78. Lendenmann, U., and J.C. Spain, 1996. 2-Aminophenol 1,6-dioxygenase: a novel aromatic ring-cleavage enzyme purified from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  79. Davis, J.K., C.C. Somerville and J.C. Spain, 1996. Cloning of hydroxylaminobenzene mutase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  80. Nadeau, L.J. and J.C. Spain, 1996. Oxidation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane (p,p’-DDT) by Alkaligienes eutrophus A5. Annual Meeting American Society for Microbiology.
  81. Driesbach, J., S. Nilsen, C. Shelley, and J. Spain, 1996. Biodegradation of 4-halophenols by Arthrobacter sp. Annual Meeting American Society for Microbiology.
  82. Nishino, S.F., and J.C. Spain, 1996. Degradation of 2,6-dinitrotoluene by bacteria. Annual Meeting American Society for Microbiology.
  83. Somerville, C.C., and J.C. Spain, 1996. Purification of a novel nitroreductase from Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  84. Schenzle, A., H. Lenke, J.C. Spain, and H.-J. Knackmuss, 1996. 3-Hydroxylaminophenol mutase from Alcaligenes eutrophus JMP134 catalyzes a Bamberger-type rearrangement. Annual Meeting American Society for Microbiology.
  85. Fiorella, P.D., and J.C. Spain, 1996. Transformation of TNT by Pseudomonas pseudoalcaligenes JS45. Annual Meeting American Society for Microbiology.
  86. Nishino, S.F., and J.C. Spain, 1995. The effect of 2,6-dinitrotoluene on 2,4-dinitrotoluene degradation. Annual Meeting Society for Environmental Toxicology and Chemistry.
  87. Parales, R.E., J.V. Parales, D. An, A. Kumar, S.M. Resnick, J.C. Spain, and D.T. Gibson, 1995. 2-Nitrotoluene dioxygenase from Pseudomonas sp. Strain JS42: Protein purification, Molecular Biology, and Substrate Specificity. American Society for Microbiology International Symposium on Pseudomonas.
  88. Shah, M.M., and J.C. Spain, 1995. Reduction of the explosive 2,4,6-trinitrophenylmethylnitramine (tetryl) catalyzed by oxygen sensitive nitro reductase enzymes. Nitration Symposium, National Meeting American Chemical Society.
  89. Haigler, B.E., and J.C. Spain, 1995. Purification and characterization of 4-methyl-5-nitrocatechol oxygenase from Pseudomonas sp. strain DNT. Annual Meeting American Society for Microbiology.
  90. Nishino, S.F., and J.C. Spain, 1995. Degradation of mixtures of nitroaromatic compounds by a gram positive bacterium. Annual Meeting American Society for Microbiology.
  91. Vorbeck, C., H. Lenke, J.C. Spain, and H.-J. Knackmuss, 1994. Initial steps in aerobic metabolism of 2,4,6-trinitrotoluene (TNT) by a Mycobacterium sp. Annual Meeting, American Society for Microbiology.
  92. An, D., D.T. Gibson, W. Wallace, and J.C. Spain, 1994. Oxidative release of nitrite from 2-nitrotoluene by a three-component enzyme system from Pseudomonas sp. strain 2NT. Annual Meeting, American Society for Microbiology.
  93. Klecka, G., S. McDaniel, P. Wilson, C. Carpenter, J. Clark, A. Thomas, and J.C. Spain, 1994. Bioreactor treatment of chlorobenzene in contaminated groundwater: results of a site demonstration. Annual Meeting, American Institute of Chemical Engineers
  94. Nadeau, L.J., and J.C. Spain, 1994. The bacterial degradation of m-nitrobenzoic acid. Annual Meeting, American Society for Microbiology.
  95. Nishino, S.F., and J.C. Spain, 1994. Oxidative pathway for the degradation of nitrobenzene by a gram-positive bacterium. Annual Meeting, American Society for Microbiology.
  96. Suen, W.C., B.E. Haigler, and J.C. Spain, 1994. 2,4-Dinitrotoluene dioxygenase genes from Pseudomonas sp. strain DNT. Annual Meeting, American Society for Microbiology.
  97. Somerville, C.C., S.F. Nishino, and J.C. Spain, 1994. Purification and characterization of nitrobenzene reductase from Pseudomonas pseudoalcaligenes, JS45. Annual Meeting, American Society for Microbiology.
  98. Wallace, W.H., D.E. Miller, and J.C. Spain, 1993. Biodegradation of chlorobenzene by indigenous microorganisms and Pseudomonas sp. JS150 in an aboveground fixed-film bioreactor. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  99. Nishino, S.F., and J.C. Spain, 1993. Isolation of nitrobenzene-degrading bacteria from contaminated sites. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  100. Nishino, S.F., and Spain, J.C., 1993. Initial steps in the mineralization of nitrobenzene by a Pseudomonas pseudoalcaligenes. Annual Meeting, American Society for Microbiology.
  101. Vogel, C.M., G. Klecka, A. Thomas, and J.C. Spain, 1993. Biodegradation of chloroaromatic compounds in aboveground bioreactors. Annual Meeting American Chemical Society.
  102. Jain, R.K., J.H. Driesbach, and J.C. Spain, 1993. Biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an Arthrobacter sp. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  103. Jain, R.K., Suen, W.C., and Spain, J.C., 1993. Cloning and expression of 2,4-dinitrotoluene degrading genes from a Pseudomonas sp. R34 in Escherichia coli. Annual Meeting, American Society for Microbiology.
  104. Reardon, K.F., and Spain, J.C., 1993. Immobilized cell bioreactor for 2,4-dinitrotoluene degradation. American Chemical Society National Meeting.
  105. Pettigrew, C.A., J.C. Spain, S.F. Nishino, and W. Wallace. 1993. Biodegradation of groundwater contaminants by indigenous and specific chlorobenzene-degrading bacteria. Bioremediation Symposium, Soil Science Society of America.
  106. Reardon, K.F., and Spain, J.C., 1993. Immobilized cell bioreactor for 2,4-dinitrotoluene degradation. Second International Symposium on In Situ and On-Site Bioreclamation.
  107. Haigler, B.E., and Spain, J.C., 1993. Degradation of 4-nitrotoluene and 4-nitrobenzoate by a Pseudomonas species. Annual Meeting, American Society for Microbiology.
  108. Suen, W.C., and Spain, J.C., 1993. Nucleotide sequence and overexpression of 2,4,5-trihydroxytoluene oxygenase gene from Pseudomonas sp. strain DNT in Escherichia coli. Annual Meeting, American Society for Microbiology.
  109. Wallace, W.H., Haigler, B.E., and Spain, J.C., 1993. Bacterial degradation of 2-nitrotoluene. Annual Meeting, American Society for Microbiology.
  110. Suen, W.C., and J.C. Spain, 1992. Cloning and characterization of Pseudomonas sp. strain DNT genes for 2,4-dinitrotoluene degradation. American Chem. Soc. Ninth International Biotechnology Symposium.
  111. Spain, J.C., S.F. Nishino, and C.A. Pettigrew, 1992. Biodegradation of groundwater contaminants by indigenous organisms. Annual Meeting, Society of Environmental Toxicology and Chemistry.
  112. Spain, J.C., S.F. Nishino, and B.F. Haigler, 1992. Biodegradation of nitroaromatic compounds. Bat-Sheva de Rothchild Foundation Symposium on Use of Natural and Genetically Engineered Microorganisms to Combat Pollution. Tel Aviv University, Israel.
  113. Haigler, B.E., S.F. Nishino and J.C. Spain, 1992. Bacterial degradation of 2,4-dinitrotoluene. National Meeting, American Society of Microbiology.
  114. Nishino, S.F., and J.C. Spain, 1992. Initial steps in the bacterial degradation of 1,3-dinitrobenzene. National Meeting, American Society of Microbiology.
  115. Robertson, J.B., and J.C. Spain, 1991. Oxidation of nitrotoluenes by toluene dioxygenase. Annual Meeting, Society for Environmental Toxicology and Chemistry.
  116. Haigler, B.E., S.F. Nishino, J.C. Spain, and R.J. Spanggord, 1991. Metabolic pathway for bacterial degradation of 2,4-dinitrotoluene. Gordon Research Conference on Applied and Environmental Microbiology.
  117. Nishino, S.F., R.J. Spanggord, and J.C. Spain. 1991. Initial steps in the bacterial degradation of 2,4-dinitrotoluene. National Meeting American Society for Microbiology.
  118. Haigler, B.F. and J.C. Spain. 1991. Degradation of nitrobenzene by bacteria containing toluene degradative pathways. National Meeting American Society for Microbiology.
  119. Pettigrew, C.A., J.C. Spain, and C.M. Vogel. 1991. Biological treatment of groundwater contaminated with mixtures of aromatic compounds. International Symposium on In Situ and On Site Bioreclamation.
  120. Henley, M.K., and J.C. Spain. 1991. Thermospray LC-MS of microbiologically important acids. Pittsburg Conference American Chemical Society.
  121. Spain, J.C. and S.F. Nishino. 1990. Metabolite accumulation serves as intercellular signal for induction of enzymes of p-nitrophenol degradation pathway. ASM International Conference on Multicellular Behavior of Bacteria. Woods Hole MA.
  122. Pettigrew, C.A., B.E. Haigler, and J.C. Spain. 1990. Simultaneous biodegradation of chlorobenzene and toluene by a Pseudomonas strain. Annual Meeting, American Society for Microbiology.
  123. Nishino, S.F. and J.C. Spain. 1990. Adaptation to biodegradation of p-nitrophenol depends on accumulation of a metabolite. Annual Meeting, American Society for Microbiology.
  124. Haigler, B.E., C.A. Pettigrew, and J.C. Spain. 1990. Multiple pathways for the biodegradation of substituted benzenes in a single strain of Pseudomonas. Annual Meeting, American Society for Microbiology.

Presentations

  1. 2015 Peking University, College of Urban and Environmental Sciences
  2. 2015 Tsinghua University, College of Hydraulic and Hydroelectric Engineering
  3. 2015 University of Minnesota, Department of Biochemistry
  4. 2015 Duke University, School of Civil and Environmental Engineering
  5. 2015 University of Science and Technology, Beijing, Department of Environmental Engineering
  6. 2015 Institute of Microbiology, Chinese Academy of Sciences, Beijing
  7. 2015 Society for Industrial Microbiology and Biotechnology, Philadelphia
  8. 2015 Battelle International Bioremediation Symposium, Miami
  9. 2014 Division Lecture, American Society for Microbiology Annual Meeting, Boston
  10. 2014 Nanjing Agricultural University, Department of Microbiology
  11. 2014 Nanjing University, School of the Environment
  12. 2014 Shanghai Jiao Tong University, School of Life Science and Biotechnology
  13. 2014 Xi’ an Jiao Tong University, School of Energy and Power Engineering
  14. 2013 University of Sydney, Department of Molecular Biology
  15. 2013 Rice University, Department of Civil and Environmental Engineering
  16. 2012 International Plant and Animal Genome Conference, San Diego
  17. 2012 University of Guelph, University Consortium for Field-Focused Groundwater Contamination
  18. 2012 University Consortium for Field-Focused Groundwater Contamination Research, Denver, CO
  19. 2012 International Plant and Animal Genome Conference, San Diego
  20. 2011 Society for Industrial Microbiology, Annual Meeting, New Orleans
  21. 2011 CSIRO Ecosystem Sciences/Australia National University, Canberra
  22. 2011CSIRO Land and Water, Perth, Australia
  23. 2011 Symposium on Microbiology of Extremophiles, University of Georgia
  24. 2011 Indo-Swiss Collaboration in Biotechnology. Recent Trends in Developing Bioremediation Strategies for Hexachlorocyclohexane and other Chlorinated Contaminants, Delhi, India
  25. 2011 University of South Australia/CRC-CARE, Adelaide, Australia
  26. 2010 American Chemical Society, Chemistry for Peace, CeRMACS, Dayton, OH
  27. 2010 International Environmental Research Center Symposium, Gwangju, Korea
  28. 2010 Unified Korean Society for Microbiology, Seoul, Korea
  29. 2010 Georgia State University, Department of Chemistry
  30. 2010 University of Georgia, Department of Microbiology
  31. 2009 Proctor and Gamble
  32. 2009 University of Iowa, Department of Microbiology
  33. 2009 American Society for Microbiology, Annual Meeting, Proctor and Gamble Award lecture
  34. 2007 Society for Industrial Microbiology, Annual meeting, Denver, CO
  35. 2007 Novozymes
  36. 2007 University of Florida, Department of Microbiology
  37. 2007 Rutgers University, Department of Environmental Sciences
  38. 2006 University of Puerto Rico, Biology Department, Mayaguez
  39. 2005 DoD Strategic Environmental Research and Development Program/Environmental Security Technology Certification Program Partners Symposium, Washington, DC
  40. 2004 American Chemical Society Symposium on: The Interface of Polymers and Biomimetics
  41. 2004 American Society for Microbiology Symposium on Biodegradation and Biotransformation
  42. 2004 Korean Society for Applied Biological Chemistry, Boryung City, Korea
  43. 2004 Federation of Korean Microbiological Societies, Seoul, Korea
  44. 2004 Second International Environmental Research Workshop, Gwangju Institute of Science and Technology, Gwangju, Korea
  45. 2004 Gordon Conference on Biocatalysis
  46. 2004 Georgia Institute of Technology, Department of Civil and Environmental Engineering
  47. 2004 University of Vermont, Microbiology & Molecular Genetics
  48. 2003 Gordon Conference on Applied and Environmental Microbiology
  49. 2003 DuPont Chemical Corporation
  50. 2003 Georgia Institute of Technology, Department of Biology
  51. 2002 Superfund Symposium: Transitioning Basic Science into Practical Applications to Meet Environmental and Public Health Challenges. Tucson, Arizona
  52. 2002 America Society for Microbiology Symposium on Microbial Oxygenases
  53. 2002 U.S. Army Environmental Center Technology Focus Meeting on Munitions
  54. 2002 Genencor International, Biocatalysis Group
  55. 2002 Symposium on Utilization of Microbes for Development of Bioremediation Technologies. Delhi, India
  56. 2002 University of Texas, School of Public Health, Houston, Texas
  57. 2002 Marine Biological Laboratory, Woods Hole, MA
  58. 2002 Cornell University, Department of Microbiology
  59. 2002 University of California—Berkeley, Department of Engineering
  60. 2002 University of Houston, Department of Engineering
  61. 2002 University of British Columbia, Department of Microbiology
  62. 2001 DoD Strategic Environmental Research and Development Program Symposium
  63. 2001 Institute of Microbial Technology, Chandigarh, India
  64. 2001 Microbial Biotechnology Department, India Habitat Center, New Delhi
  65. 2001 ASM/SGM Conference on Biodegradation, Biotransformation, and Biocatalysis, San Juan, Puerto Rico
  66. 2001 Workshop on Microbes for the Environment: Degradation of Xenobiotics and New Benign Products, Bad Honnef, Germany
  67. 2001 NATO Workshop on Environmental Remediation Technology Developments, Prague, Czech Republic
  68. 2001 Society for General Microbiology, Edinburgh, Scotland
  69. 2001 University of Tennessee, Department of Microbiology
  70. 2001 Biology Department, University of Delhi
  71. 2000 Microbial Ecology and Environmental Engineering, Lawrence Berkeley National Laboratory
  72. 2000 Genencor International, Biocatalysis Group
  73. 2000 Department of Energy Savannah River Laboratory
  74. 2000 CSIC Department of Biochemistry and Cell Biology, Granada, Spain
  75. 2000 University of Konstanz, Germany
  76. 2000 Institute of Biotechnology, ETH Zurich
  77. 2000 FOA Defense Research Establishment, Umea, Sweden
  78. 1999 Second International Symposium on Biodegradation of Nitroaromatic Compounds and Explosives, Leesburg, Virginia
  79. 1999 U.S.-Canada-Australia Trilateral Meeting on Environmental Impacts of Energetic Materials
  80. 1999 Searle/Monsanto Bioprocess/Biocatalysis Group
  81. 1999 European Congress on Biotechnology, Brussels
  82. 1999 Battelle International Bioremediation Symposium, San Diego
  83. 1999 University of California, Riverside Department of Chemical and Environmental Engineering
  84. 1999 Colorado State University, Department of Agricultural and Chemical Engineering
  85. 1999 University of Wyoming, Departments of Engineering and Microbiology
  86. 1999 University of Stuttgart, Institute for Microbiology
  87. 1998 University of Iowa, Biocatalysis and Bioprocessing Conference.
  88. 1998 University of Tennessee, Department of Microbiology
  89. 1998 Center for Environmental Biotechnology, Lawrence Berkeley National Laboratory
  90. 1997 National Environmental Technology Applications Center Conference on Innovative Groundwater Remediation, Philadelphia, PA
  91. 1997 ICI Nobel Explosives, Ardeer, Scotland
  92. 1997 Air Products and Chemicals, Industrial Chemicals Division
  93. 1997 DuPont Chemical, Environmental Biotechnology Group
  94. 1997 A.H. Marks Chemical Co. Ltd. Bradford, England
  95. 1997 Battelle International Bioremediation Symposium
  96. 1997 UK Soil and Groundwater Association Annual Meeting, Torbay, England
  97. 1997 Gordon Research Conference on Applied and Environmental Microbiology
  98. 1997 American Society for Microbiology-International Conference on Pseudomonas Biology, Vancouver, BC
  99. 1997 Swiss Federal Institute for Water Resources and Pollution Control, Zurich
  100. 1997 Los Alamos National Laboratory, Environmental Biotechnology Division
  101. 1997 Stanford University, Department of Civil and Environmental Engineering
  102. 1996 U.S. EPA-Air Force Symposium on Natural Attenuation of Chlorinated Organics in Groundwater Dallas, Texas
  103. 1996 Department of Energy Savannah River Laboratory
  104. 1996 Fraunhofer Institute for Applied Microbiology, Stuttgart, Germany
  105. 1996 European Environmental Research Organization Symposium on Biodegradation of Pollutants, Palma Majorca, Spain
  106. 1996 Engineering Foundation Conference on Bioremediation of Surface and Subsurface Contamination, Palm Coast, FL
  107. 1996 American Society for Microbiology Symposium on Biodegradation and Biotransformation of Aromatic Compounds, New Orleans, LA
  108. 1996 Swiss Federal Institute for Water Resources Research, Duebendorf, Switzerland
  109. 1995 Society for Industrial Microbiology Symposium on Bioremediation Science and Technology, San Jose, CA
  110. 1995 International Symposium on Biochemical Engineering at the University of Stuttgart, Germany
  111. 1995 Juan March International Workshop on the Molecular Basis for Biodegradation of Pollutants. Madrid, Spain
  112. 1995 Microbial Ecology Forum, U.S. EPA, Gulf Breeze, FL
  113. 1995 Agriculture and Biotechnology Center, Rutgers University
  114. 1995 Keynote speaker, Texas A&M University, Bioremediation Workshop
  115. 1994 Pfizer Central Research Division Environmental Sciences Section
  116. 1994 Department of Microbiology, University of Iowa
  117. 1994 Keynote Speaker, Colorado Biotechnology Symposium
  118. 1993 U.S. Army Construction Engineering Research Laboratory- sponsored Workshop on Bioremediation of Explosives at Northwestern University
  119. 1993 Molecular Biology and Environmental Research Division, E.I. DuPont Corporation
  120. 1993 Imperial Chemical Industries, Ardeer, Scotland
  121. 1993 ICI/DuPont-Sponsored Workshop on Biodegradation of Nitroaromatic and Explosive Chemicals
  122. 1993 Celgene Environmental Biotechnology Group
  123. 1993 Department of Environmental Science and Engineering, Rice University
  124. 1993 Department of Microbiology, University of Georgia
  125. 1993 Department of Microbiology, University of Nebraska
  126. 1992 Department of Microbiology, Ohio State University
  127. 1992 University of Notre Dame Center for Bioengineering and Pollution Control-Hazardous Waste Conference
  128. 1992 Invited lecture at the WE-Heraeus Seminar on Microbial Degradation of Environmental Pollutants, Bad Honnef, Germany
  129. 1992 Session chairman and invited lecture at The Second Environment Science and Technology Congress, Bangkok, Thailand
  130. 1992 National Research Council of Canada Biotechnology Research Institute, Montreal Canada
  131. 1992 International Conference on Bioinorganic and Biotechnological Aspects of Environmental Chemistry sponsored by the European Environmental Research Organization, Florence, Italy
  132. 1991 Departments of Agricultural and Chemical Engineering, Colorado State University
  133. 1991 U.S. Environmental Protection Agency, Environmental Research Center, Cincinnati, OH
  134. 1991 National Meeting American Society for Microbiology
  135. 1991 Environmental Biotechnology Symposium, University of Tennessee.
  136. 1990 Los Alamos National Laboratory, Environmental Biotechnology Division
  137. 1990 American Society for Microbiology Conference on Biotechnology
  138. 1990 American Society for Microbiology National Meeting, Symposium on "Cooxidation of Organic Compounds"
  139. 1990 Society for Environmental Toxicology and Chemistry.
  140. 1989 University of Tennessee, Department of Microbiology
  141. 1989 American Society for Microbiology International Conference on Pseudomonas Biology, Biotechnology, and Applications
  142. 1989 American Chemical Society National Meeting Symposium on "Advances in Enhanced Soil Microbial Degradation Research"
  143. 1989 American Chemical Society National Meeting Symposium on "Metabolic Pathway Engineering"
  144. 1988 Engineering Foundation Conference on Biotechnology Applications in Hazardous Waste Treatment
  145. 1988 Annual Meeting of the American Society for Microbiology Symposium on "Microbiological Degradation of Chlorinated Aromatic and Aliphatic Compounds"

Updated date: September 12, 2019 - 15:53